Multiplexing based on ALPN is deprecated

The use of port 443 for both jitsi meet and turn server has been deprecated in the latest release.

Does this require (turns) port 5349 to be opened in the firewall?

no, just disable output to ports 10000 and 5349 on your computer and connect to with 2 other devices and you will see that it works. Or disable only port 10000 and check port 5349 with a network scanner such as wireshark.