Audio is not working with atleast 2 users

Hello All,
We have installed it in the aws - ubuntu 18.04 server. Given the own wildcard SSL, and successfully mapped to a domain. We will able to access it and run the meeting. But the audio is not working. Is there any configuration to set in the server, please let me know,

Reference: Which I have tried to install

Ports enabled:
HTTP https 80,443,
TCP 4443, 5222, 5347, 5280
UDP 10000 - 20000

But we didn’t get the audio. Please help me with this.